biotin labeled anti crth2 Search Results


biotin anti crth2 rea598  (Miltenyi Biotec)
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Biotin Anti Crth2 Rea598, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti crth2 biotin  (Thermo Fisher)
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Thermo Fisher anti crth2 biotin
Anti Crth2 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotin-conjugated crth2  (Miltenyi Biotec)
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Biotin Conjugated Crth2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4oc with anti crth2 biotin  (Miltenyi Biotec)
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anti crth2 primary antibody  (Miltenyi Biotec)
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Miltenyi Biotec anti crth2 primary antibody
Fig. 2. DHA treatment does not inhibit <t>CRTh2</t> (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Anti Crth2 Primary Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rorγ (t) antibody, anti-human/mouse, reafinity  (Miltenyi Biotec)
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Miltenyi Biotec rorγ (t) antibody, anti-human/mouse, reafinity
Fig. 2. DHA treatment does not inhibit <t>CRTh2</t> (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Rorγ (T) Antibody, Anti Human/Mouse, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd25 antibody, anti-human  (Miltenyi Biotec)
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Miltenyi Biotec cd25 antibody, anti-human
Fig. 2. DHA treatment does not inhibit <t>CRTh2</t> (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Cd25 Antibody, Anti Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-cd45ro biotinylated mab  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc anti-cd45ro biotinylated mab
Fig. 2. DHA treatment does not inhibit <t>CRTh2</t> (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Anti Cd45ro Biotinylated Mab, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe anti- crth2  (Becton Dickinson)
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Becton Dickinson pe anti- crth2
Fig. 2. DHA treatment does not inhibit <t>CRTh2</t> (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Pe Anti Crth2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg2a  (Serotech Inc)
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Serotech Inc rat igg2a
Fig. 2. DHA treatment does not inhibit <t>CRTh2</t> (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Rat Igg2a, supplied by Serotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti biotin pe  (Miltenyi Biotec)
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Miltenyi Biotec anti biotin pe
Fig. 2. DHA treatment does not inhibit <t>CRTh2</t> (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Anti Biotin Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-biotin-apc  (Becton Dickinson)
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Becton Dickinson anti-biotin-apc
Fig. 2. DHA treatment does not inhibit <t>CRTh2</t> (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)
Anti Biotin Apc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 2. DHA treatment does not inhibit CRTh2 (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)

Journal: British Journal of Nutrition

Article Title: n-3 Fatty acids inhibit transcription of human IL-13: implications for development of T helper type 2 immune responses

doi: 10.1017/s0007114512002917

Figure Lengend Snippet: Fig. 2. DHA treatment does not inhibit CRTh2 (chemoattractant receptor-homologous molecule expressed on T helper type 2 cells) on human Th2 cells. Repre- sentative flow cytometry staining of CRTh2 on human Th2 cells cultured for 4 d in (a) IL-2 ( ) or IL-2 þ DHA ( , 10 mM) and shows there was no reduction in (b) the percentage of CRTh2þ cells (n 4). Statistical significance was assessed by Student’s t test. (A colour version of this figure can be found online at http://www.journals.cambridge.org/bjn)

Article Snippet: Chemoattractant receptor-homologous molecule expressed on T helper type 2 cells and T helper type 2 cytokine staining Surface staining of CRTh2 was performed at 4 8C by first blocking (30 min) with normal rat IgG (Invitrogen; Life Technologies), then addition (30 min) of anti-CRTh2 primary antibody (CRTh2-biotin; Miltenyi Biotech) or isotype (rat IgG2a; ABD Serotech) followed by addition (30 min) of Streptavidin-APC (eBiosciences).

Techniques: Cytometry, Staining, Cell Culture

Fig. 5. DHA treatment inhibits transcription factor binding to the IL-13 promoter. Electromobility shift assay with nuclear extracts from phorbol myristate acetate (PMA) (20 ng/ml)- and ionomycin (1 mM)-stimulated (a) Jurkat and (b) CRTh2þTh2 cells show the binding of cyclic AMP response element binding (CREB) and activating transcription factor 2 (ATF2) to the IL-13 promoter. Nuclear extracts from CRTh2þTh2 cells stimulated in the presence of (c) DHA (10 mM) show that the binding of both CREB and ATF2 is reduced. Data are representative of four experiments. CRTh2, chemoattractant receptor-homologous molecule expressed on T helper type 2 cells; Ab, antibody.

Journal: British Journal of Nutrition

Article Title: n-3 Fatty acids inhibit transcription of human IL-13: implications for development of T helper type 2 immune responses

doi: 10.1017/s0007114512002917

Figure Lengend Snippet: Fig. 5. DHA treatment inhibits transcription factor binding to the IL-13 promoter. Electromobility shift assay with nuclear extracts from phorbol myristate acetate (PMA) (20 ng/ml)- and ionomycin (1 mM)-stimulated (a) Jurkat and (b) CRTh2þTh2 cells show the binding of cyclic AMP response element binding (CREB) and activating transcription factor 2 (ATF2) to the IL-13 promoter. Nuclear extracts from CRTh2þTh2 cells stimulated in the presence of (c) DHA (10 mM) show that the binding of both CREB and ATF2 is reduced. Data are representative of four experiments. CRTh2, chemoattractant receptor-homologous molecule expressed on T helper type 2 cells; Ab, antibody.

Article Snippet: Chemoattractant receptor-homologous molecule expressed on T helper type 2 cells and T helper type 2 cytokine staining Surface staining of CRTh2 was performed at 4 8C by first blocking (30 min) with normal rat IgG (Invitrogen; Life Technologies), then addition (30 min) of anti-CRTh2 primary antibody (CRTh2-biotin; Miltenyi Biotech) or isotype (rat IgG2a; ABD Serotech) followed by addition (30 min) of Streptavidin-APC (eBiosciences).

Techniques: Binding Assay, Electro Mobility Shift Assay